Analysis Note

ControlTreated versus untreated A431 cells

Application

Milli-Mark Anti-Phosphotyrosine-PE Antibody, recombinant clone 4G10 is an antibody against Phosphotyrosine clone 4G10 for use in FC.

General description

Some of the tyrosine residues can be tagged with a phosphate group by protein kinases and in its phosphorylated state, it is referred to as phosphotyrosine. Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of the significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosine phosphorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyne phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified mouse monoclonal IgG2bk conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by flow cytometry using EGF treated versus untreated A431 cells

Specificity

Expected to detect tyrosyne phosphorylated proteins in all species.

Antibody recognizes tyrosine-phosphorylated proteins from all species

Target description

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.